Concentration calculator

Reconstitution math for BPC-157, expressed as weight/volume percent (1% w/v equals 10 mg/mL).

What this calculates

Weight/volume percent describes how much solid is dissolved in a given volume of liquid: a 1% w/v solution is one gram of material in 100 mL, which is the same thing as 10 mg/mL. The calculator multiplies your target percentage by your final volume to give the mass of BPC-157 that volume requires.

Worked through: a 2% w/v solution at 30 mL needs 2% of 30 mL expressed as mass — 0.6 g, or 600 mg. Halve the volume and you halve the mass; the concentration is unchanged. This is arithmetic about a solution, not a recommendation about a quantity.

What to check before preparing BPC-157

• Starting material. White to off-white lyophilised powder, stated under FDA's physicochemical characterisation heading for BPC-157 (free base). FDA describes BPC-157 acetate separately as a white to off-white solid powder. source
• Solubility. Soluble in water at 5 mg/mL. PROVENANCE: FDA states this in its characterisation section for both the free base and the acetate, but footnotes it to supplier product pages rather than to a published measurement. Because the substance is water-soluble and would be solubilised before administration, FDA does not consider particle size a critical quality attribute for the solution dosage forms it reviewed. source A target concentration above what the compound will dissolve produces a suspension, not a solution, and the calculator has no way to know that — it does the arithmetic either way.
• Storage. READ THE PROVENANCE BEFORE USING THIS. FDA's evaluation reports that lyophilised BPC-157 (free base) is stable at room temperature for three weeks, that it is recommended to be held desiccated below -18 degrees C because exposure to moisture greatly decreases the long-term stability of lyophilised peptides, and that once reconstituted it is stable for two to three weeks at 4 degrees C and for three to four months at -20 degrees C. FDA's own footnotes attribute every one of those figures to supplier product pages, not to a published stability study, and FDA states the conclusion as what is "reported in the literature" rather than as an agency determination. No peer-reviewed forced-degradation or shelf-life study for BPC-157 was located. FDA separately warns that peptides such as this one are extremely sensitive to formulation, process and environmental conditions including pH, temperature, concentration and excipients, which may lead to aggregation and degradation and to loss of biological activity, and that significant amounts of aggregates can form during storage. source

What the number does not tell you

The result assumes the vial contains the mass its label states and that the material is what the label says it is. Neither is established by arithmetic. Both come from an assay — what HPLC and mass spectrometry establish — and the published record on BPC-157 has limits worth reading before treating any figure as settled.